SAPPHIRE 800 CE Detector for Capillary Electrophoresis
Programmable wavelength adjusting 190 up to 800nm, application for capillary electrophoresis.
SAPPHIRE CE is programmable detector derived from basic unit SAPPHIRE UV-VIS Variable Wavelength DETECTOR equipped with originally designed optics (with quartz optical fibers) permitting the application of spectrophotometric detection directly on a separation capillary. To do the operating easily is the reading unit assembled on front panel of unit. The reading unit is connected to the detector by an electrical cable (which leads the signals from preamplifier) and by an optical fiber cable which leads a monochromatic light beam from monochromator to the center of quartz capillary for electrophoresis.
These units have excellent features as follows:
- High sensitivity, especially in the range of short wavelengths (200-230nm)
- Large linear range
- Very good access and simple replacement of a separation capillary
Capillary assembly in to reading unit:
Technical parameters:
| Diameter of optical fiber cable | 0.2 mm |
| Diameter of quartz capillary | 0.375 mm |
| Max. length of optical fiber cable by external assembly | 0.5 - 1 m |
| Dimensions | 36 x 55 mm |
Warning: The reading unit works with very low light value. There it is necessary to prevent the preamplifier from daily light coming in. If the daily light infiltrate along the capillary, put on it the black tubing, or use a black sticky tape to caver the slits.
Capillary electrophoresis is a modern separation method for analysis or micro preparation of substances. Separation of components in a solution occurs due to effects of an electric field in a quartz capillary. An advantage of capillary electrophoresis when compared with HPLC consists in particular in miniaturization of the separation area, in higher analysis speed, higher separation efficiency, higher selectivity, etc.
Note: This unit design is in exclusive property of ECOM. All use including reproduction and publication, without prior written consent of ECOM, is prohibited.
Technical parameters:
| Parameter | Value (Conditions) |
| Wavelength range | 190 - 800 nm |
| Spectral half-width | 6 nm |
| Accuracy of adjustment | ± 1 nm |
| Reproducibility | ± 0.5 nm |
| Light source | Deuterium discharge lamp and tungsten lamp |
| Noise level at empty cell (240 nm, TC 1 s) | ± 0.5 x 10-5 AU |
| Drift at empty cell (240 nm after 1 h) | 1 x 10-4 AU/hr |
| Diameter of used Fused Silica Capillary Tubing | ID 0.1 / OD 0.363 |
| Time constant | Fast, middle and slow |
| Programming | 3 change of wavelength during analysis |
| Adjustable offset | ± 50 mV |
| Output for integrator | 1 V/AU |
| Interface | RS 232 |
| Power supply | 100, 115 and 230, 50/ 60 Hz |
| Power input | 80 VA |
| Dimensions (W x H x D) | 220 x 170 x 450 mm |
| Weight | 11 kg |
