ECOM Separpur PRO is a universal system for separation and purification of proteins and peptides by high-performance & high-pressure preparative liquid chromatography. The system includes everything needed for the successful separation of proteins and peptides and their identification. It allows performing all known separation methods: absorption chromatography (mostly in reversed-phase mode), affinity chromatography, size-exclusion chromatography, and ion exchange chromatography.
ECOM Separpur PRO allows performing separations at room and at elevated temperatures but also at temperatures close to zero, which is needed when cleaning particularly sensitive substances. The system can run high-performance chromatography column packed with microparticles (10 µm up to 15 µm) because of its high pump pressure (250 bar). System triplex pump PP03 is characterized by a compact stainless steel head with 3 pistons and pulse-less flow. Thanks to the stepping motor it has a wide range of flow rates and allows the use wide range of column diameters starting with micropreparation columns (I.D. 10 mm) up to almost industrial-scale ones with I.D. 150 mm.
On the pump head entry is installed a gradient mixing unit equipped with five-fast solenoid valves with PEEK-made bodies. Three of them are used to produce a gradient, remaining two can be programmed for an on-off function according to a given schedule.
ECOM Separpur PRO exploits an excellent UV-VIS detector DAD 12, working parallel on four wavelengths and making fast spectral scans. The detector is compatible with a 3D module of the Clarity data station (part of the supply) upholding the best possible overview of purity and uniformity of fraction. There are two next submodules for monitoring the pH and conductivity of the mobile phase on column output. Monitors are characterized by high sensitivity and their data are automatically corrected on temperature dependence.
ECOM Separpur PRO hardware is divided into three parts, which are placed in identical stainless steel-made cabinets with rounded edges and sloping front panels (upper and bottom). In the bottom cabinet is located the pump; the UV detector, gradient valves, and a passive mixing cell are located in the middle one. On the slanted front panel of this module are mounted a loop injection valve, a bypass valve, and a valve for reversing the flow through the column. The panel allows easy installation of hydraulic connections among elements or the installation of new ones.
Chromatograph ECOM Separpur PRO allows working with medium or low-pressure columns as well - which are typical for older methods of protein purification.
Nevertheless, the use of high-efficiency microparticular sorbents increases purifying performance as the high molecular weight of proteins is characterized by a low diffusion rate into sorbent particles.
ECOM Separpur PRO can be completed either by rotating fraction collectors separflow FFC 96. In the case of a large range of separations, separpur FC 5-5 or separpur FC 5 -10 are recommended.